ZooCell Lecture and Practical course

ZooCELL volume EM Course

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ZooCell Network

Animal diversity and EM sample preparation

Programme.

03-14 February 2025, COS, Heidelberg

These lectures are organised by the ZooCELL Doctoral Network and are open to all members of COS.

ZooCELL is a Marie Sklodowska-Curie Doctoral Network that will train 12 doctoral candidates in a new field of comparative integrative cell biology in animals. The network brings together 8 beneficiaries and 7 associated partners, located in 6 different countries (France, Germany, Italy, Netherlands, Sweden and UK) and one intergovernmental organisation (EMBL-ZooCELL coordinator). 12 Doctoral candidates will enjoy a multi-disciplinary and international environment with plenty of training opportunities and exchange with all labs involved in the Network.

Day 1 - Mon. 03.02

Morning lectures (Seminar room 007 in INF230)

11:00 - 11:45: Lecture. “Sample preparation for EM imaging” by Karel Mocaer, COS.

Evening lectures

18:30 - 19:30: PI talks (SR041 Bioquant)

  • “Cell type dynamics across sea urchin development: focus on Paracentrotus lividus nervous system”. Ina Arnone. SNZ. Italy.
  • “Perfect regeneration of a complex organ: exploring leg regeneration in Parhyale”. Michalis Averof. IGFL. France.

Day 2: Tue. 04.02

Morning lectures (Seminar room 007 in INF230)

09:00 - 10:00: Lecture. “Overview of volume EM methods” by Réza Shahidi, EMCF.

10:00 - 10:45: Lecture. “Image formation in EM” by Alexandra Kerbl, COS.

Evening lectures

18:30 - 19:30: SR041 Bioquant

  • “tbc”. Max Telford. UCL. United Kingdom.
  • “Similarly specialized cells of Clytia and coral larval aboral ends” Richard Copley. IMEV. France.

Day 3: Wed. 05.02

Morning lectures (Seminar room 007 in INF230)

09:00 - 10:00: Lecture. “Volume correlations: vCLEM, CLXEM” by Yannick Schwab, EMBL

10:00- 10:45: Lecture. “Large vEM and connectomics” by Gáspár Jékely, COS.

10:45 - 11:00: Coffee break.

11:00 - 11:30: Lecture. “Alternative imaging methods_X-ray tomography” by Angelika Svetlove. EMBL Hamburg. Germany.

11:30 - 12:00: Lecture. “Alternative imaging methods_Expansion microscopy” by Gautam Day. EMBL Heidelberg. Germany .

Evening lectures

18:30 - 19:30: COS INF230 005 Seminar room

  • “Larval settlement and metamorphosis in the polychaete Platynereis dumerilii (and beyond?!)” Elizabeth Williams. UNEXE. United Kingdom.
  • tbc Evelyn Houliston. IMEV. France.

Day 4: Thu. 06.02

Morning lectures (Seminar room 007 in INF230)

09:00 - 10:45: Lecture. “Overview of Animal Diversity” by Andreas Hejnol, University of Jena

Evening lectures

18:30 - 19:30: COS INF230 005 Seminar room

  • “Building bilaterian brains: Coregulons and morphofeatures”. Detlev Arendt. EMBL. Germany.
  • “Ctenophores, ultrastructure and an embryonic organizer” Andreas Hejnol and Nadia Rimskaya- Korsakova. FSUJENA. Germany.

Day 5: Fri. 07.02

Evening lectures

18:30 - 19:45: COS INF230 005 Seminar room.

  • “Cell-type atlas and neuronal connectome of the Platynereis dumerilii 3d larva” Gáspár Jékely. COS. Germany.
  • “The ancestry of the Lophotrochozoa plus Grube Messel: an introduction” Graham Budd and Ralf Janssen. UU. Sweden.

19:30- 19:45: Introduction to the Messel Pit fossil site by Graham Budd

Day 8: Mon. 10.02

Morning lectures (Seminar room 007 in INF230)

11:00 - 12:00: Sustainable scientific practices by EMBL sustainability team.

Evening lectures

18:30 - 19:30: PI talks (005 Seminar room)

  • “Machine Learning for EM image analysis: from segmentation to morphology”. Anna Kreshuk. EMBL Heidelberg. Germany.
  • “tbc”Yannick Schwab. EMBL Heidelberg. Germany.

Day 9: Tue. 11.02

Morning lectures (Seminar room 007 in INF230)

10:00 - 10:45: “Immuno EM: overview of the methods” by Réza Shahidi, EMCF

10:45 - 11:00: Coffee break

11:00 - 12:00: Introduction Spatial transcriptomics by Marco Grillo, SU (007 Seminar room_virtual)